The Impact of Tinospora Cordifolia Dry Seed Water Extract on the Phagocytic Activity of Microglia

Fuchangfeng Liu

doi:10.54254/2753-8818/2025.AU25641

Theoretical and Natural ScienceOpen access

The Impact of Tinospora Cordifolia Dry Seed Water Extract on the Phagocytic Activity of Microglia

Research Article

Open Access

The Impact of Tinospora Cordifolia Dry Seed Water Extract on the Phagocytic Activity of Microglia

Fuchangfeng Liu ^1*

¹ The International Curriculum Center of the High School Affiliated to Renmin University of China, Beijing, China

^*Corresponding author: liufuchangfeng@outlook.com

Published on 30 July 2025

TNS Vol.133

ISSN (Print): 2753-8826

ISSN (Online): 2753-8818

ISBN (Print): 978-1-80590-303-1

ISBN (Online): 978-1-80590-304-8

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Abstract

Evaluating the potential mediating effects of Tinospora cordifolia (TC) extracts on microglial phagocytosis is necessary to research on the therapeutic efficacy of this rarely studied plant on neurodegenerative diseases associated with underregulated microglial phagocytic activity. Research has demonstrated the extract’s impact on human neutrophil cells; however, experiments are still in need to include microglia, immune cells in the central nervous system that are tightly bound with various diseases. This research used the Trypan Blue exclusion test to evaluate the cytotoxicity of TC dry seed water extract’s influence on the viability of mouse microglia Bv2 cells, and adopted pH-sensitive fluorescent microspheres to evaluate the impact of various concentrations of TC extract on the phagocytic activity of microglia as measured by relative fluorescent units. Results indicate that 0.1 μg/ml – 1.0 μg/ml TC dry seed water extract did not show significant effects on both cell viability and microglial phagocytosis, while 2.0 μg/ml TC dry seed water extract demonstrated the ability to reduce microglial phagocytosis without exhibiting influential cytotoxicity. 5.0 μg/ml TC dry seed water extract revealed strong cytotoxicity and was therefore not tested subsequently. In conclusion, 2.0 μg/ml TC dry seed water extract serves as a potential agent for managing the phagocytic activity of microglia under inflammation, and therefore may be experimented with for further medical use.

Keywords:

Microglial phagocytosis, Tinospora cordifolia, Cytotoxicity

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[3]. Richard M. Ransohoff & Melissa A. Brown. (2012). Innate immunity in the central nervous system. Journal of Clinical Investigation, 122(4), 1164–1171. https: //doi.org/10.1172/JCI58644

[4]. Suzanne Hickman, Izzy, S., Sen, P., Morsett, L., & El Khoury, J. (2018). Microglia in neurodegeneration. Nature Neuroscience, 21(10), 1359–1369. https: //doi.org/10.1038/s41593-018-0242-x

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Cite this article

Liu,F. (2025). The Impact of Tinospora Cordifolia Dry Seed Water Extract on the Phagocytic Activity of Microglia. Theoretical and Natural Science,133,1-8.

Data availability

The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.

About volume

Volume title: Proceedings of ICBioMed 2025 Symposium: AI for Healthcare: Advanced Medical Data Analytics and Smart Rehabilitation

ISBN: 978-1-80590-303-1(Print) / 978-1-80590-304-8(Online)

Editor: Alan Wang

Conference website: https://2025.icbiomed.org/auckland.html

Conference date: 17 October 2025

Series: Theoretical and Natural Science

Volume number: Vol.133

ISSN: 2753-8818(Print) / 2753-8826(Online)

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